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Part:BBa_K648103:Design

Designed by: Elyse Merkel   Group: iGEM11_Penn_State   (2011-09-28)


RecA with mutation of Arg 243


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 127
    Illegal AgeI site found at 631
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Mutate Arg 243 because it is known to affect the recombinase of damaged DNA, which trying to prevent this strain of RecA of doing without changing its other functions


Source

RecA1 Dh10B

References